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Optical Metabolic Imaging

Optical Metabolic Imaging (OMI) is a technique to gain information from the autofluorescent properties and the fluorescent lifetimes of reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD). Particularly the optical redox ratio, defined by the NADH fluorescence intensity devided by the FAD fluorescence intensity, is calculated and compared with the FLIM images of NADH and FAD. This technique provides information about the cellular metabolism.

Photo: Fluorescence lifetime Image of a melopsittacus undulatus feather recorded by the multiphoton tomograph MPTflex

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