K. Reinhard, H.G. Breunig, K. König. Autofluorescence lifetime variation in the cutucle of the bedbug Cimex lectularius. Arthropod Structure & Development 46(2017)56-62

M. Balu et al. In vivo multiphoton-microscopy of picosecond-laser-induced optical breakdown in human skin. Lasers in Surgery and Medicine(2017)Doi: 10.1002/lsm.22655


M.E. Darvin et al. Comparison of morphologic criteria for actinic keratosis and squamous cell carcinoma using in vivo multiphoton tomography. Exp Dermatol 25(2016)218

Batista, H.G. Breunig, K. König, A.M. Morgado, A. Uchugonova. Two-photon spectral fluorescence lifetime and second harmonic generation imaging of the porcine eye with a 12 femtosecond laser microscope. JBO 21(3)(2016)036002

A. Giese et al. In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue. J Neurooncol online(2016 Jan 30)

M.A. Fallah et al. From morphology to biochemical state-intravital multiphoton fluorescence lifetime imaging of inflamed human skin. Sci. Rep. 6(2016)


H.G. Breunig, K. König, M. Weinigel. In vivo imaging of ZnO Nanoparticles from sunscreen on human skin with a mobile multiphoton tomograph. BioNanoScience 5(2014)42

K. König. Skin problems in outer space. New Economy Spring Edit(2015)

M. Balu et al. In vivo multiphoton microsocpy of basal cell carcinoma. JAMA Dermatol online (2015 April 24)

H.G. Breunig, K. König. Two-photon imaging of intact living plants during freezing with a flexible multiphoton tomograph. Laser Phys Lett 12(2015)025601

Anderson, H.G. Breunig, K. König, T. Liu.Multiphoton imaging with a novel compact diode-pumped Ti:sapphire oscillator.Microsc Res Technol 78(2015)1154

H.G. Breunig, K. König, A. Uchugonova, M. Weinigel. Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology. J Med Imaging 2(2015)0160031

H.G. Breunig et al. Impact of refractive index mismatches on coherent anti-Stokes Raman scattering and multiphoton autofluorescence tomography of human skin in vivo. Phys in Med & Bio 60(2015)6881

H.G. Breunig, K. König, K. Reinhardt, A. Uchugonova. Sperm metabolism is altered during storage by females: evidence from fluorescence lifetime measurements in bedbugs. J. R. Soc. Interface 12(2015)

M.E. Darvin, K. König, M. Kaatz, J. Lademann, S. Springer, M. Zieger. Optimization of the measurement procedure during multiphoton tomography of human skin in vivo. Skin Res Tech 0(2015)1

K. König. Leading the way in innovation and research. New Economy Spring Edit(2015)


H.G.Breunig, K. König, J. Lademann, M. Weinigel. In vivo histology: optical biopsies with chemical contrast using multiphoton/CARS tomography. Laser Phys Lett 11(2014)055601

M. Balu et al. Distinguishing between benign and malignant melanocytic nevi by in vivo multiphoton microscopy. Cancer Res 74(2014)2688

K. König. Optical biopsies with femtosecond lasers. New Economy Summer Edit(2014)44

M.E. Darvin et al. Comparison of in vivo and ex vivo laser scanning microscopy and multiphoton tomography application for human and porcine skin imaging. Quant Electro 44(7)(2014)646

K. König. Multiphotonen-Tomographie der humanen Kornea. Ophthalm Nach Sept(2014)


F. Arginelli et al. Diagnosis of BCC by multiphoton laser tomography. Skin Res Tech 19(2013)297

H.G. Breunig, K. König, F. Tümer. Multiphoton imaging of freezing and heating effects in plant leaves. J Biophoton online (2012 Sep 14)

F. Arginelli et al. High-resolution imaging of basal cell carcinoma: a comparison between multiphoton microscopy with fluorescence lifetime imaging and reflectance confocal microscopy. Skin Res Tech 19(2013)433

Alex et al. Three-dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology. J Biophoton online(2013 Jun 18)

H.G. Breunig et al. Clinical coherent anti-stokes Raman scattering and multiphoton tomography of human skin with femtosecond laser and photonic crystal fiber. Laser Phys Lett 10(2013)025604

F. Arginelli et al. High resolution diagnosis of common nevi by Multiphoton Laser Tomography and Fuorescence Lifetime Imaging. Skin Res Tech 19(2013)194

M. Balu et al. In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin. Biophys J 104(2013)258

M.E. Darwin, K. König, J. Lademann, M.C. Meinke, M. Ulrich. Comparison of in vivo refelectance confocal microscopy and multiphoton microscopy to detect basal cell carcinoma. J Biomed Optics 18(2013)61229

K. König, R. Hoffmann, A. Uchugonova. Multiphoton tomography visualizes collagen fibers in the tumor microenvironment that maintain cancer cell anchorage and shape. Cell Biochem 114(2013)99

Azzoni et al. High-resolution multiphoton tomography and fluorescence lifetime imaging of UVB-induced cellular damage on cultured fibroblasts producing fibres. Skin Res and Tech 19(2103)251

R.M. Hoffman, K. König, A. Uchugonova. Stem cell imaging in living animals. G.I.T. Imag&Micros 3(2103)44

Campagnola, C.H Dong, K. König, J. Mertz, P.T.C So, C. Xu. Multiphoton Microscopy: Technical innovations, biological applications, and clinical diagnostics. J Biomed Opt 18(2013)3


Hoffman et al. Cancer-cell killing by engineered Salmonella Imaged by multiphoton tomography in live mice. Anticancer Res 32(2013)4331

F. Arginelli et al. Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin. Dermatol Res Pract Vol (2012)810749

H.G. Breunig, R. Bückle, M. Kellner-Höfer, K. König, J. Lademann, W. Sterry, M. Weinigel. Combined In Vivo Multiphoton and CARS Imaging of Healthy and Disease-Affected Human Skin. Microsc Res Tech 75(2012)492

H.G. Breunig et al. Safety Assessment by Multiphoton Fluorescence/ Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products. Skin Pharmacol Physiol 25(2101)219

Elsner et al. Non-invasive evaluation of dermal elastosis by in vivo multiphoton tomography with autofluorescence lifetime measurements. Exp. Dermatol 1(2012)48

Alexandrov et al. Multispectral fluorescence lifetime inaging using multiphoton tomography for the evaluation of basal cell carcinomas. PloS ONE 7(9)(2012)

K. König. Hybrid Multiphoton Multimodal Tomography of In Vivo Human Skin. Intravital 1(2012)8

F. Arginelli et al. Multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma: morphological features for non-invasive diagnostics. Exp Dermatol 21(2012)831


H.G. Breunig et al. Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography. Laser Phys Lett 8(2011)1

W. Becker et al. Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy.
Eur J Pharma and Biopharma 77(2011)469

P. Elsner et al. Keratinocyte morphology of human skin evaluated by in vivo multiphoton laser tomography. Skin Res and Tech 17(2011)479

S. Astner et al. Clinical application of multiphoton tomography in combination with confocal laser scannig microscopy for evaluation of skin diseases. Skin Res and Tech 20(2011)589

J. Duong, R.M. Hoffman, K. König, A. Uchugonova, N. Zhang. The bulge area is the origin of nestin-expressing pluripotent stem cells of the hair follicle. J Cell Biochem 112(2011)2046

M. Hoffman, K. König, A. Uchugonova, M. Weinigel. Watching stem cells in the skin of living mice non-invasively. Cell Cycle 10(2011)2017

H.G. Breunig et al. Applications of multiphoton tomographs and femtosecond laser nanoprocessing microscopes in drug delivery research. Adv Drug Deliv Res. 63(2011)388

V. Bellini et al. Quantitative evaluation of healthy epidermis by means of multiphoton microscopy and fluorescence lifetime imaging microscopy. Skin Res Tech April(2011)

H.G. Breunig et al. Application of ultrafast gold luminescence to measureing the instrument response function for multispectral multiphoton fluorescence lifetime imaging.
Optics Express 19(2011)13848

Y. Alexandrov et al. Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels. Biomed Optics Express 2(12) online(2011 Nov 10)3295


R. Bückle et al. Depth-resolved measurement of the dermal matrix composition by multiphoton laser tomography. Skin Res and Tech 16(2010)131

M. Kaatz, K. König. Multiphotonenmikroskopie und In-vivo-Multiphotonentomographie in der dermatologischen Bildgebung. Der Hautarzt 61(2010)397

H.G. Breunig, K. König, H. Studier. Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo. Opt Exp 18(2010)7857

R. Bazin et al. Clinical study on the effects of a cosmetic product on dermal extracellular matrix components using a high-resolution multiphoton tomograph. Skin Res and Tech 16(2010)305

R. Bückle et al. In vivo measurement of the human epidermal thickness in different localizations by multiphoton laser tomography. Skin Res and Tech 16(2010)259

M. Kaatz, M.J. Köhler, K. König, R. Scharenberg, M. Speicher. Clinical application of multiphoton tomography in combination with high-frequency ultrasound for evaluation of skin diseases. J Biophoton 3(2010)759


E. Dimitrow et al. Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis. Exp Dermatol 18(2009)509

R. Bückle et al. Clinical optical coherence tomography combined with multiphoton tomography of patients with skin diseases. J Biophoton 2(2009)389

R. Bückle et al. Intrinsic, solar and sunbed-induced skin aging measured in vivo by multiphoton laser tomography and biophysical methods. Skin Res and Tech 15(2009)357

E. Dimitrow et al. Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma. J Invest Dermatol 129(2009)1752


K. König. Clinical Multiphoton Tomography. J Biophoton 1(2008)13

A. Bauer et al. Morphological skin ageing criteria by multiphoton laser scanning tomography: non-invasive in vivo scoring of the dermal fibre network. Exp Dermatol 17(6)(2008)519

R. Bückle et al. Multiphoton tissue imaging using high-NA microendoscopes and flexible scan heads for clinical studies and small animal research. J Biophoton 1(2008)506


R. Bückle et al. Clinical two-photon microendoscopy. Microsc Res and Tech 70 (5)(2007)398


A. Ehlers, K. König, I. Riemann, F. Stracke. In vivo drug screening in human skin using femtosecond laser multiphoton microscopy. Skin Pharmacol Physiol 19(2)(2006)78

K. König et al. Multiphoton microscopy for the investigation of dermal penetration of nanoparticle-borne drugs. J Invest Dermatol 126(2006)2224

R. Bückle, P. Elsner, M. Kaatz, M. J. Köhler, K. König. In vivo assessment of human skin aging by multiphoton laser scanning tomography. Opt Lett 31(19)(2006)2879

A. Ehlers et al. Influence of nanoencapsulation on human skin transport of flufenamic acid. Skin Pharmacol Physiol 19(4)(2006)190

O. Damour, K. König, K. Schenke-Layland, I. Riemann, U. A. Stock. Two-photon microscopes and in vivo multiphoton tomographs – novel diagnostic tools for tissue engineering and drug delivery.  Adv Drug Deliv Rev: Multiphoton Imaging: Diseases and Therapies 58(7)(2006)878


K. König et al. Multiphoton autofluorescence imaging of intratissue elastic fibers. Biomat 26(2005)49


U. Hintze et al. Dead but highly dynamic – the stratum corneum is devided into three hydration zones. Skin Pharmacol Physiol 17(2004)246


K. König, I. Riemann. High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. J Biomed Opt 8 (3)(2003)432














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